HPLC UV Method for Simultaneous Analysis of Glyphosate and N-(Phosphonomethyl)iminodiacetic acid (PMIDA) on Newcrom BH Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of PMIDA (Phosphonomethyliminodiacetic acid) , Glyphosate .

Glyphosate is a broad-spectrum herbicide. It works through inhibiting the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase. The enzyme is essential for producing amino acids within the plant. Glyphosate is used across agriculture and forestry, as well as rare aquatic environments. It’s chemical formula is C3H8NO5P . You can find detailed UV spectra of Glyphosate and information about its various lambda maxima by visiting the following link.

N-(Phosphonomethyl)iminodiacetic acid , also known as PMIDA , is a key intermediate in the making of glyphosate. It has the chemical formula (HO) 2 P(O)CH2N(CH2CO2H) 2 . You can find detailed UV spectra of PMIDA and information about its various lambda maxima by visiting the following link.

PMIDA (Phosphonomethyliminodiacetic acid) , Glyphosate can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.