HPLC Method for Simultaneous Determination of Lactic Acid, Taurine, and Myo-Inositol on Primesep S2 Column
Separation type: Liquid Chromatography HILIC
Commonly used in food preservation, cosmetics, pharmaceuticals, and as a biomarker in physiological studies (e.g., exercise and metabolism).
Widely present in energy drinks and supplements; important in cardiovascular function, development of the nervous system, and cellular protection.
Used in the treatment of polycystic ovary syndrome (PCOS), neurological conditions, and as a dietary supplement for metabolic and reproductive health.
The mixture of lactic acid, taurine, and myo-inositol can be effectively retained and separated using a mixed-mode Primesep S2 column with a mobile phase consisting of 80% acetonitrile (MeCN) , without the need for a buffer.
The mixture consists of lactic acid, an organic α-hydroxy acid; taurine, a sulfonic acid amino acid derivative; and myo-inositol, a polyol (sugar alcohol), representing diverse chemical classes with varying polarities and functional groups. This method offers high resolution and excellent peak symmetry , enabling accurate quantification of all three analytes. Detection is performed using ELSD (Evaporative Light Scattering Detection) , which provides reliable sensitivity for non-UV-active or weakly absorbing compounds.
Methodenparameter
| Column | Primesep S2, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN80% |
| Buffer | none |
| Flow Rate | 1.0 mL/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |