HPLC Separation of β-Lactam Antibiotics such as Amoxicillin and Cephalexin on Primesep 100 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Cephalexin , Amoxicillin , Amoxicillin hydrate (1:3) .
Both amoxicillin and cephalexin are beta-lactam antibiotics. Beta-lactam antibiotics contain a beta-lactam ring in their molecular structure. As a group, these drugs are active against many gram-positive, gram-negative and anaerobic organisms. Amoxicillin is a prescription antibiotic with the molecular formula C16H19N3O5S . It is typically used to treat bacterial infections and is taken orally. Cephalexin has the chemical formula C16H17N3O4S .
Cephalexin , Amoxicillin , Amoxicillin hydrate (1:3) can be retained and baseline separated with an isocratic method in 30/70 Acetonitrile (ACN) and water mobile phase with a Sulfuric acid (H2SO4) buffer. UV detection at 250 nm.
Methodenparameter
| Column | Primesep 100, 4.6 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O |
| Buffer | TFA, H2SO4 |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 250 nm |