HPLC Separation of Albuterol and Amoxicillin
Primesep 100 separates albuterol and amoxicillin with baseline resolution by a combination of cation-exchange, hydrophobic, and polar interactions. The mass spec (LC/MS) compatible separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with UV detection at 210 nm.
Methodenparameter
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | TFA – 0.15% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 210 nm |