HPLC Separation of Antibiotics of Group in Mixed-Mode Chromatography
Separation type: Liquid Chromatography Mixed-mode
Primesep D was used to separate the antibiotics marbofloxacin, norfloxacin, josamycin, and flumequine. Primesep D is a reverse-phase column with embedded basic ion-pairing groups designed for analysis of plasma. This m ethod is compatible with LC/MS and prep chromatography.
Methodenparameter
| Column | Primesep D, 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | Gradient MeCN – 5-50%, 10 min, 3 min hold |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 250 nm |