Analysis of Antibiotic Flumequine and Related Impurities in Mixed-Mode Chromatography

The antibiotic Flumequine is typically used in veterinary medicine. Flumequine and related impurities have been found in animals later sold for meat. Analysis was performed by by mixed-mode chromatography on Primesep D, a reverse-phase column with embedded basic ion-pairing groups and Obelisc R with multiple separation modes (RP,NP,IE).

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.