Analysis of Antibiotic Norfloxacin and Related Impurities in Mixed-Mode Chromatography
The antibiotic norfloxacin is a first generation fluoroquinolone. It is effective against both gram positive and gram negative bacteria. Analysis was done on Obelisc R and Primesep D.
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Methodenparameter
| Column | Obelisc R, 2.1×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | Gradient MeCN – 5-30%, 10 min, 5 min hold |
| Buffer | Gradient Formic Acid – 0.05%-0.3%, 10 min, 5 min hold |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 270 nm |