Applikationen / HPLC Separation of Organic Acids in HILIC and Anion-Exclusion Mode on Primesep S2 Column

HPLC Separation of Organic Acids in HILIC and Anion-Exclusion Mode on Primesep S2 Column

HPLC-Applikation • Datum: 2011/07/14
HPLC Separation of Organic Acids in HILIC and Anion-Exclusion Mode on Primesep S2 Column Chromatogramm

Organic acids were separated on a HILIC/cation-exchange column in HILIC/anion-exclusion mode. This column can be used for analysis of polar compounds in HILIC mode. If compounds are ionizable, additional mode of interaction can be added (cation-exchange or anion-exclusion).

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Methodenparameter

ColumnPrimesep S2, 4.6×150 mm, 5 µm, 100 Å
Mobile PhaseMeCN/H2O – 85/15%
BufferAmAc pH 5.0 15 mM
Flow Rate1.0 mL/min
DetectionELSD, 50C UV 250 nm

Direktzugriff

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