HPLC Separation of Organic Acids Glucuronic Acid, Methanesulfonic Acid, and Butanesulfonic Acid on Primesep SB Column
The organic acids glucuronic acid, methanesulfonic acid, and butanesulfonic acid were separated using Primesep SB mixed-mode column. Primesep SB is a reverse-phase column with embedded basic ion-pairing groups.
Methodenparameter
| Column | Primesep SB, 4.6×50 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | Gradient AmFm pH 3.0- 2-50 mM, 3 min, 3 min hold |
| Flow Rate | 1.0 mL/min |
| Detection | CAD |