HPLC Separation of Mixture of DMF and DMSO
High Performance Liquid Chromatography (HPLC) Method for Analysis of DMF (Dimethylformamide) , DMSO (Dimethyl sulfoxide) .
Dimethylformamide, DMF, is primarily used as an organic solvent and is miscible with water and most other organic liquids. Dimethyl sulfoxide (DMSO) is also a solvent. It can dissolve both polar and nonpolar compounds and is miscible with water and other organic liquids. Both compounds can be retained and separated using anhydrous (water-free) conditions using HPLC by SHARC1column, which uses hydrogen-bonding as a separation mechanism. The method uses a gradient of acetonitrile (ACN) and methanol (MeOH) mobile phase without the need for a buffer. UV detection used at 220 nm.
Methodenparameter
| Column | SHARC1, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 98-85%, 10 min |
| Buffer | No |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 220 nm |