HPLC Method for Separation of a Mixture of Tryptophan and its Catabolites on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode
Tryptophan and its catabolites participate in several biological pathways, having roles in protein synthesis, serving as precursors to bioactive molecules, and influencing several physiological processes. Here’s an overview considering a mixture of tryptophan and its catabolites:
Tryptophan and its Catabolites can be retained, separated and analyzed on a Primesep 100 mixed-mode stationary phase column using an gradient analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid as a buffer. This analysis method can be detected using UV at 220 nm.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN5-50% in 10 min, with 2 min hold afterwards |
| Buffer | Gradient H2SO4from 0.1-0.2% in 10 min, with 2 min hold afterwards |
| Flow Rate | 1.0 mL/min |
| Detection | UV 220 nm |