HPLC Separation of Mixture of 2-Hydroxyethyl Salicylate and Salicylic Acid on Primesep B Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Hydroxyethyl salicylate , Salicylic acid
2-Hydroxyethyl salicylate is a drug used as analgesic agents for the treatment of mild to moderate pain.
2-Hydroxyethyl salicylate , Salicylic acid can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep B |
|---|---|
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 238 nm |