HPLC Separation of Mixture of Atropine, Atrolactic Acid and Pralidoxime Chloride
High Performance Liquid Chromatography (HPLC) Method for Analysis of Atropine , Atrolactic Acid , Pralidoxime Chloride .
Atropine is a tropane alkaloid with the chemical formula C17H23NO3 . It is an anticholinergic medication that is used to treat nerve agent, pesticide poisoning, slow heartrate, and is also used to decrease saliva production. Typically, it is administered through injection.
Atrolactic Acid is a 2-hydroxy monocarboxylic acid with the chemical formula C9H10O3 . It can cause irritation to eyes, skin, and the respiratory system.
Pralidoxime Chloride , also known as 2-PAM, is a cholinesterase reactivator with the chemical formula C7H9N2O + . It is used as an antidote for poisoning by organophosphate pesticides and nerve agents.
Atropine , Atrolactic Acid , Pralidoxime Chloride can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 200, 4.6 x 150 mm, 3 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | Gradient H3PO4 – 0.1-0.3%, 10 min |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 200 nm |