HPLC Separation of Mixture of Carbocisteine, Methylparaben & Propylparaben on Primesep 100 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Carbocisteine , Methylparaben sodium , Propylparaben sodium , Propylparaben , Methylparaben .
Carbocysteine is a medication that reduces the viscosity of mucus making it easier to cough up. Methylparaben and propylparaben are used as preservatives in cosmetics, pharmaceuticals and food. They can be separated in HPLC on a Primesep 100 mixed-mode column by both hydrophobic and ionic properties present on the stationary phase. The analytical method uses acetonitrile (ACN) gradient and water with sulfuric acid (H2SO4) as buffer and UV detected at 200 nm.
Methodenparameter
| Column | Primesep 100 |
|---|---|
| Mobile Phase | Gradient MeCN – 2-50%, 20 min |
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 200 nm |