HPLC Separation of Glyphosate, Glyphosine, Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric Acids on Newcrom B Column

HPLC-Applikation • Datum: 2019/12/04
HPLC Separation of Glyphosate, Glyphosine,  Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric Acids on Newcrom B Column Chromatogramm

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylphosphonic Acid , Methylphosphonic Acid , Glyphosate , Glyphosine , Methylenediphosphonic acid , Phosphate , Phosphoric Acid .

Glyphosate is an herbicide with a chemical formula of C3H8NO5P . It works through blocking enzymes, like 5-enolpyruvylshikimate-3-phosphate synthase, essential for plant growth. It is typically found in agricultural work, but can occasionally be found in forestry and garden care.

Glyphosine is a synthetic plant growth regulator with the chemical formula of C4H11NO8P2 . It is a colorless liquid that works through decreasing chlorophyll production.

Ethylphosphonic acid has a C2H5P(O)(OH) 2 chemical formula. It is typically found as white crystals or crystalline powder. It is often used as an internal standard when researching fosfomycin in human plasma as well as a synthetic nucleotide analog.

Methylphosphonic acid is an organophosphorus compound with the chemical formula CH3P(OH) 2 . It is often used in some lubricant additives, textile treatments, and in synthesis of phosphonate compounds, like the previously mentioned Glyphosate.

Methylenediphosphonic acid has the chemical formula CH 2 [P(O)(OH) 2 ] 2 . It is typically seen as a precursor in synthesis of Mesoporous aluminum organophosphate, if alkyltrimethylammonium, and Tetraester of methylenediphosphonic acids.

Methodenparameter

ColumnNewcrom B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended
Mobile PhaseMeCN Gradient -40-10%, 15 min
BufferFormic Acid Gradient -1- 5%, 15 min
Flow Rate1.0 mL/min
DetectionCAD