HPLC Separation of Dicyandiamide, 1,2,4-Triazole, 3-Methylpyrazole on Primesep 100 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Dicyandiamide , 1,2,4-Triazole , 3-Methylpyrazole
Dicyandiamide, or cyanoguanidine, is used as a curing agent for epoxy resins. 1,2,4-triazole, is a heterocycle used primarily as an antifungal but has other uses in the pharmaceutical industry as well. 3-methylpyrazole is used in nitrogen fertilizers. All three compounds are structurally similar and can be separated in HPLC using Primesep 100 reverse-phase (RP) mixed-mode cation-exchange (CX) column using acetonitrile (ACN) and water mobile phase with phosphoric acid buffer and UV detected at 200 nm and 220 nm.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 10% |
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200, 220 nm |