HPLC Separation of Triazole acetic acid and 1,2,4-Triazole on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of 1,2,4-Triazole , Triazole acetic acid

Triazole is a five-membered heterocyclic chemical ring containing three nitrogen atoms and two carbon atoms. It’s molecular formula is C2H3N3 . It is most commonly used as an antifungal drug. It works through inhibiting an enzyme in fungal cell wall synthesis.

1,2,4-Triazole , Triazole acetic acid can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Trifluoroacetic acid buffer. Detection is performed using CAD.