HPLC Separation of Coumaric acids on Newcrom BH Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of m-Coumaric acid , o-coumaric acid , p-coumaric acid .
p -Coumaric acid is a major phenolic acid with the chemical formula C9H8O3 . It can be found in a wide variety of graminaceous plants. p -Coumaric acid is a natural metabolite contained in many edible plants and its antioxidant activities in reducing oxidative stress and inflammatory reactions have been demonstrated in various experiments.
m -Coumaric acid is a hydroxycinnamic acid with the chemical formula C9H8O3 . It can be found in a vinegar. It differs from p -Coumaric acid in the position of the hydroxy substitution.
o -Coumaric acid is a hydroxycinnamic acid with the chemical formula C9H8O3 . It can be found in a vinegar. It differs from p -Coumaric acid and m-Coumaric acid in the position of the hydroxy substitution.
The three isomers of coumaric acid, m-Coumaric acid , o-coumaric acid , p-coumaric acid , can be detected at low UV. Using Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN), and water with phosphoric acid (H3PO4) buffer, the acids can be retained and UV detected at 270 nm.
Methodenparameter
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H3PO4 – 1.0% |
| Flow Rate | 1.0 mL/min |
| Detection | 270 nm |