HPLC Separation of Active Compounds in Drug Formulation
An HPLC method for the separation of active drug compounds on a Primesep 200 column. The retention of compounds is achieved through reverse-phase, cation exchange and hydrophobic interactions. Benzoic acid, hyoscyamine sulfate and phenyl salicylate are baseline separated using simple mobile phases of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with a UV detector.
Methodenparameter
| Column | Primesep 200, 3.0×50 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O |
| Buffer | TFA |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 220 nm |