HPLC Separation of Agricultural Compounds Found in Tea
Tea has been known to contain residue of pesticides and fungicides such as picoxystrobin and pyrimethanil. We used Primesep 200, a reversed-phase column with weakly acidic ion-pairing groups to separate picoxystrobin, pyrimethanil and atorvastatin. A 15 minute method used to demonstrate retention control when adjusting ratio of organic to aqueous in the mobile phase. Baseline retention with good resolution was achieved.
Methodenparameter
| Column | Primesep 200, 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 45/55%, MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 250 nm |