HPLC Separation of Acetaminophen, Phenylephrine, and Diphenhydramine
Primesep C separates acetaminophen (paracetamol), phenylephrine, and diphenhydramine– common over-the-counter cold and flu medication ingredients such as Tylenol products—by either an acetonitrile or phosphoric acid gradient. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and phosphoric acid with ultraviolet (UV) detection at 210 nm.
Methodenparameter
| Column | Primesep C, 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN |
| Buffer | H3PO4 |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 210 nm |