HPLC Separation of 2-HEP and 2-HPP in the Presence of Methylcobalamin and Amino Acids
Methylcobalamin, S-Adenosyl-L-methionine, 2-hydroxyethyl phosphonic acid, and 2-hydroxy propyl phosphonic acid were separated in reversed-phase anion-exchange mixed-mode on a Primesep SB HPLC column. Compounds are monitored by UV and ELSD. Method is compatible with LC/MS. Selectivity of separation is controlled by varying the amount of ACn buffer pH and buffer concentration.
Methodenparameter
| Column | Primesep SB, 4,6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN – 20% |
| Buffer | AmFm |
| Flow Rate | 1.0 mL/min |
| Detection | ELSD35C, UV 250 nm |