HPLC MS Method for Analysis of Vitamin E ((±)-α-Tocopherol) and Vitamin E Acetate (α-Tocopheryl Acetate) on Lipak Column

(±)-α-Tocopherol is a form of vitamin E, a fat-soluble antioxidant that protects cell membranes from oxidative damage by neutralizing free radicals. Widely found in dietary sources like nuts and vegetable oils, it plays a critical role in maintaining skin health, immune function, and overall cellular protection. α-Tocopheryl acetate, on the other hand, is a stable esterified form of α-tocopherol. Commonly used in dietary supplements and skincare products, it is converted to active α-tocopherol in the body, offering a prolonged shelf life and enhanced stability in formulations.

Vitamin E , Vitamin E Acetate can be retained, and analyzed using a Lipak mixed-mode stationary phase column. The analysis utilizes an gradient method with a mobile phase consisting of water, methanol (MeOH), ammonium formate and formic acid as a buffer. Detection is achieved using UV 292 nm or MS.