HPLC Method For Analysis of Ergocalciferol (Vitamin D2) and Cholecalciferol (Vitamin D3) on Primesep SB Column
Ergocalciferol ( Vitamin D2 ) and Cholecalciferol ( Vitamin D3 ) are fat soluble vitamins that help your body absorb calcium and phosphorus. Your body can produce both when exposed to the sun, but you can also intake these essential vitamins through a daily multivitamin or a Vitamin D pill.
Vitamins D2 and D3 can be detected in the high UV regime. Using a Primesep SB reverse-phase column and a mobile phase consisting of water and acetonitrile with an acetic acid buffer, Vitamins D2 and D3 can be retained and separated. This analysis method can be UV detected at 265 nm.
Methodenparameter
| Column | Primesep SB, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 80/20% |
| Buffer | Acetic Acid – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 265 nm |