Analysis of Nicotinic Acid and Nicotinamide (Vitamin B3) on Newcrom AH Column
Nicotinamide is the amide form of nicotinic acid. Nicotinic acid is in the vitamin B group and is converted to nicotinamide in vivo. Newcrom AH was used to separate nicotinamide and nicotinic acid. Newcrom AH separates acids using ion-exclusion, retains basic compounds by cation-exchange, and neutral compounds by reverse-phase mechanism.
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A , AH , B , and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Methodenparameter
| Column | Newcrom AH, 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 210 nm |