HPLC Method for Analysis of Thymol Blue on Primesep 100 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol blue
Thymol Blue is a sulfonephthalein dye with C27H30O5Smolecular formula. It is typically used as a pH indicator. Despite it’s name, it is only blue at higher pH levels, turning from blue to yellow when going basic to neutral and from yellow to red when changing from relatively acidic to very acidic. Due to the split in transition stages, it us considered a diprotic acid.
Thymol blue can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | Formic Acid – 0.3% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 270 nm |