HPLC Method for Analysis of Brilliant Blue FCF and Allura Red in Monster Energy Ultra Violet, Sugar Free Energy Drink
High Performance Liquid Chromatography (HPLC) Method for Analysis of Brilliant Blue FCF (Blue 1) , Allura Red (Red #40) .
Brilliant Blue FCF (Blue 1) is a synthetic molecule used as a food coloring agent. Allura Red (Red 40) is another food colorant. Both are present in Monster Energy Ultra Violet sugar free drink. Both are disodium salts and can be separated in HPLC by our Newcrom BH mixed-mode column which utilizes both hydrophobic and ion-exchange properties. The mobile phase is methanol (MeOH) and water with sulfuric acid (H2SO4) buffer making the method MS-compatible. Can also be UV detected at 210 nm
You can find detailed UV spectra of Blue 1 and information about its various lambda maxima by visiting the following link.
You can find detailed UV spectra of Red 40 and information about its various lambda maxima by visiting the following link.
Methodenparameter
| Column | Newcrom BH, 4.6 x 50 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeOH/H2O – 75/25% |
| Buffer | H2SO4 – 0.5%% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 210 nm |