HPLC Method for Analysis of Sucralose, Mannitol, and Lactulose on Primesep S2 Column Using ELSD
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sucralose , Mannitol , Lactulose
Sucralose is an synthetic organochlorine compound with the molecular formula C12H19Cl3O8 .
Properties: Appearance: Typically a white, odorless, crystalline powder.
Molecular weight: ~397.6 g/mol
Solubility: Soluble in water.
Uses: An artificial sweetener used to provide sweetness in food and beverages without the calories of sugar.
Methodenparameter
| Column | Primesep S2, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 85% |
| Buffer | Formic Acid – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | ELSD |