HPLC Method for Analysis of Mannitol on Primesep S2 Column

HPLC-Applikation • Datum: 2025/08/11
HPLC Method for Analysis of Mannitol on Primesep S2 Column Chromatogramm

High Performance Liquid Chromatography (HPLC) Method for Analysis of Mannitol

Mannitol is an organic compound with the molecular formula C6H14O6 .

Properties: Appearance: Typically a white, odorless, crystalline powder or granules.

Molecular weight: ~182.17 g/mol

Solubility: Soluble in water.

Uses: A versatile medication with various uses in healthcare, primarily as an osmotic diuretic.

Methodenparameter

ColumnPrimesep S2, 4.6 x 150 mm, 5 µm, 100 Å, dual ended
Mobile PhaseMeCN – 85%
BufferFormic Acid – 0.2%
Flow Rate1.0 mL/min
DetectionELSD
Limit of Detection4.380 ppm