HPLC Method for Analysis of Isonicotinic Acid on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
Isonicotinic Acid can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm.
You can find detailed UV spectra of Isonicotinic Acid and information about its various lambda maxima by visiting the following link .
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 10% |
| Buffer | H2SO4 -0.05% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |
| Injection Volume | 1 µl |