HPLC Method for Analysis of Isonicotinic Acid on Primesep 100 Column on Alltesta™
High Performance Liquid Chromatography (HPLC) Method for Analysis of Isonicotinic Acid
Isonicotinic Acid is an nucleoside with the C6H5NO2molecular formula. It is a derivative of pyridine, making it a pyridinemonocarboxylic acid. It acts primarily as a metabolite in humans and algae.
Isonicotinic Acid can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 30% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |