HPLC Method for Analysis of Acetylsalicylic acid (ASA) on Primesep 100 Column on Alltesta™
High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetylsalicylic Acid (Aspirin)
Acetylsalicylic acid (ASA) , also known as Aspirin , is an organic compound with the molecular formula C9H8O4 . It is a nonsteroidal anti-inflammatory drug (NSAID) that is used to reduce fever, pain, and inflammation. It is also used to treat Kawasaki disease, pericarditis, and rheumatic fever. Aspirin is a genetic medication that is widely available and can be procured without a prescription. It is one of the oldest NSAIDs in the world.
Acetylsalicylic Acid (Aspirin) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Methodenparameter
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 50% |
| Buffer | Sulfuric Acid |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |