HPLC Method for Analysis of Dibasic Acids on Newcrom B Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Adipic Acid , Succinic Acid , Glutaric acid , Methylmalonic Acid .

Succinic acid is a key intermediary (in the form of its anion, succinate) in the electron transport chain, a process that is the key power source for our cells. It also has a few limited functions outside of the mitochondria. It has the chemical formula C4H6O4 .

Glutaric acid is a common plasticizer precursor with the chemical formula C5H8O4 . It is often used in the production of polyester polyols and polyamides. Interestingly, it is significantly more water soluble than either Succinic or Adipic acid.

Adipic acid , also known as hexanedioic acid , is a key precursor to nylon, and more than 2.5 billion kilograms are produced every year. In medicinal uses, it has been incorporated into controlled-release formulation as well as the polymeric coating of hydrophilic monolithic systems. It has the chemical formula C6H10O4 .

Methylmalonic Acid is an organic acid with the chemical formula C4H6O4 . It works as an intermediate in the breakdown of certain amino acids and fatty acids. Elevated levels of Methylmalonic acid can be a sign of a Vitamin B12 deficiency or Methylmalonic acidemia, which is a rare genetic disorder where the previously mentioned acid is not properly metabolized.

Adipic Acid , Succinic Acid , Glutaric acid , Methylmalonic Acid can be separated, retained, and analyzed on a mixed-mode Newcrom B column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (FA). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.