HPLC Analysis of Fatty Acids with MS Compatible Conditions

Fatty acids are carboxylic acids with a long unbranched carbon chain. They are hydrophobic compounds with different number of carbons. Most naturally occurring fatty acids have a chain of an even number of carbon atoms. Three fatty acids are separated on a Primesep 100 HPLC column by reversed-phase mechanisms. This general HPLC method and mixed-mode column can be used for separation of other fatty acids.