HPLC Method for Analysis of Creatine and Creatinine on Chromni Column

HPLC-Applikation • Datum: 2025/09/17
HPLC Method for Analysis of Creatine and Creatinine on Chromni Column Chromatogramm

High Performance Liquid Chromatography (HPLC) Method for Analysis of Creatine , Creatinine

Creatine is a non-protein amino acid-like compound with the molecular formula C4H9N3O2 .

Properties: Appearance: Typically a white odorless powder.

Molecular weight: ~131.13 g/mol

Solubility: Soluble in water.

Uses: Dietary supplement.

Methodenparameter

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 Å, dual ended
Mobile PhaseMeCN – 70%
BufferPhosphoric Acid
Flow Rate1.0 mL/min
DetectionUV 275 nm