HPLC Method for Analysis of Colistin A on BIST B+ Column

Separation type: Bridge Ion Separation Technology, or BIST™

Colistin A is a last-resort antibiotic drug sued to treat Gram-negative bacterial infection, such as pneumonia. Using SIELC’s newly introduced BIST™ method, Colistin A can be retained on a positively-charged anion-exchange BIST™ B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged Copper and peptide to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Colistin A can be separated, retained, and UV detected at 200 nm