HPLC Method for Analysis of Chlorpheniramine Maleate on BIST B+

Chlorpheniramine Maleate , also known as Chlorphenamine , is a popular antihistamine used to provide relief from the symptoms of allergies, hay fever, and the common cold with the chemical formula C16H19ClN2 . It works through blocking histamine H1 receptor. It is said to produce less sedation than other first-generation histamines. Other side effects include drowsiness, dizziness, confusion, constipation, nausea, and more.

Using SIELC’s newly introduced BIST™ method, Chlorpheniramine Maleate, which separates in water, can be retained on a positively-charged anion-exchange BIST B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Chlorpheniramine maleate can be separated, retained, and UV detected at 210 nm and 275 nm.