HPLC Method for Analysis of Bivalirudin and Asp9 – bivalirudin on BIST A+
Bivalirudin is a short, 21-amino-acid peptide with the chemical formula C98H138N24O33 . It has 2 basic and 6 acidic groups. It is sold as a direct thrombin inhibitor (DTI), which is used as an anticoagulant.
Asp 9 -bivalirudin is a process impurity and can be a result of the degradation of the drug bivalirudin. It has the chemical formula C98H137N23O34 .
SIELC’s new BIST™ mode can retain and separate Bivalirudin and its degradant with a TMDAP phosphate buffer, and the respective retention times can be controlled by altering the pH of the buffer.
Methodenparameter
| Column | BIST A+, 2.1 x 100 mm, 3 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 30% |
| Buffer | TMDAP phosphate – 5 mM pH 5.0, pH 6.0 |
| Flow Rate | 0.2 mL/min |
| Detection | UV 275 nm |