HPLC Method for Analysis of Benzalkonium Chloride on Primesep SB Column
Separation type: Liquid Chromatography Mixed-mode
Benzalkonium chloride, also known as Zephiran or Alkyldimethylbenzylammonium chloride, is a positively charged surfactant often used as a pharmaceutical preservative and as an antiseptic. Benzalkonium Chloride can be retained on a mixed-mode stationary phase Primesep SB column with embedded strong basic ion-pairing groups, using a gradient analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) ionic modifier. This analysis method can be UV detected at 210 nm with high resolution and peak symmetry.
Methodenparameter
| Column | Primesep SB, 3.2 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 30-70%, 10 min |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.5 mL/min |
| Detection | UV 210 nm |