HPLC Method for Analysis of Adipic Acid on Newcrom B Column
Adipic acid , also known as hexanedioic acid , is a key precursor to nylon, and more than 2.5 billion kilograms are produced every year. In medicinal uses, it has been incorporated into controlled-release formulation as well as the polymeric coating of hydrophilic monolithic systems. It has the chemical formula C6H10O4 .
Adipic Acid can be retained and analyzed on a mixed-mode Newcrom B column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.
Methodenparameter
| Column | Newcrom B, 4.6 x 150 mm, 3 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O -5/95% |
| Buffer | H2SO4 – 0.02% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |