HPLC Method for Analysis of 6-Aminocaproic Acid in a High Salt Content Matrix on Primesep A Column
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
6-Aminocaproic acid (6ACA) is a synthetic amino acid that is primarily used as a medication.
6-Aminocaproic acid can be retained, separated and analyzed using a Primesep A mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and perchloric acid as a buffer. This method allows for detection using UV 200 nm.
You can find detailed UV spectra of 6-Aminocaproic acid and information about its various lambda maxima by visiting the following link .
Methodenparameter
| Column | Primesep A, 4.6 x 250 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 20% |
| Buffer | HClO4 -0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |
| Injection Volume | 10 µl |