HPLC Determination of Folic Acid on Primesep 100 Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Folic Acid .
Folic acid is a synthetic form of folate, a B vitamin, that is used as in supplements and to fortify food. Folic acid can be retained using Primesep 100 mixed-mode column, which has both hydrophobic and ion exchange properties due to embedded acidic ion-pairing groups. The analytical method uses a mobile phase of acetonitrile (ACN) and water with ammonium formate (AmFm) buffer, making the method MS-compatible. Can also use UV detection at 250 nm.
Methodenparameter
| Column | Primesep 100 |
|---|---|
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | AmFm pH 3.0- 10 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 250 nm |