HPLC Determination of Citric Acid on Newcrom B Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Citric Acid
Citric Acid is a naturally occurring organic acid found in citrus fruits; it is also an intermediate in the citric acid cycle of aerobic organisms. It is used industrially as an acidity regulator, flavoring, detergent, and more than 2 million tons are produced annually. It’s chemical formula is C6H8O7 .
Citric Acid can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using CAD.
Methodenparameter
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 1.0 mL/min |
| Detection | CAD (Corona) MS- compatible mobile phase |