HPLC Analysis of Zearalenone and Ochratoxin A on Primesep B2 Mixed-Mode Column

HPLC-Applikation • Datum: 2015/05/11
HPLC Analysis of Zearalenone and Ochratoxin A on Primesep B2 Mixed-Mode Column Chromatogramm

Zearalenone and Ochratoxin A are harmful mycotoxins commonly found in grains and other foods. They were separated using the Primesep B2 reversed-phase anion-exchange mode column. Retention can be controlled by adjusting concentration of both acetonitrile and buffer.

Methodenparameter

ColumnPrimesep B2, 4.6×150 mm, 5 µm, 100 Å
Mobile PhaseMeCN/H2O
BufferFormic Acid
Flow Rate1.0 mL/min
DetectionUV, 250 nm