HPLC Analysis of Ethephon in Hydrogen-Bonding Mode
Ethephon is an important compound for the agricultural industry. It can be corrosive, but metabolizes to ethylene quickly in plants. Ethephon was retained on SHARC1HILIC/anion-exclusion column. Retention was controlled by adjusting methanol in mobile phase to suppress hydrogen bonding.
The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column . Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.
Methodenparameter
| Column | Sharc 1, 3.2×100 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | Gradient MeCN/MeOH – 90/10% to 50/50, 6 min |
| Buffer | Ammonia – 30 mM |
| Flow Rate | 1.0 mL/min |
| Detection | ELSD |