HPLC Method for Analysis of Codeine and Guaifenesin in Cough syrup on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Codeine , Guaifenesin
Codeine is a pain reliever and cough suppressant . It belongs to the opioid family (like morphine, but milder). Guaifenesin is an expectorant , which helps loosen mucus in your chest so you can cough it up more easily.
Codeine , Guaifenesin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 270 nm.
Method Parameters
| Mobile Phase | MeCN – 30% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Codeine,Guaifenesin |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended