HPLC Method for Analysis of Insulin on BIST™B+ Column

https://sielc.com/hplc-method-for-analysis-of-insulin
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Chromatogram

Method Parameters

Mobile PhaseMeCN Gradient
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 210 nm
Class of CompoundsPolymer, Protein
Analyzing CompoundsInsulin

HPLC Column Used

BIST™B+, 4.6×150 mm, 5 µm, 100A
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