HPLC Method for Analysis of Estetrol, Estiol, Estrone, and Besta-Estradiol on Chromni Column
https://sielc.com/hplc-method-for-analysis-of-estetrol-estiol-estrone-besta-estradiolChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Estrone , Estradiol , Estetrol , Estriol
Estetrol is an synthetic organochlorine compound with the molecular formula C 18 H 24 O 4 . · Properties: Appearance: Typically a white, odorless, powder. · Molecular weight: ~270.4 g/mol · Solubility: Soluble in water. · Uses: Production of contraceptives. · Estriol is an synthetic organochlorine compound with the molecular formula C 18 H 24 O 3 .
Method Parameters
| Mobile Phase | MeCN – 20% |
|---|---|
| Buffer | Ammonium Formate – 10mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 280 nm |
| Class of Compounds | Estrogen |
| Analyzing Compounds | Estrone,Estradiol,Estetrol,Estriol |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended