HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 2 Wavelengths
https://sielc.com/hplc-method-for-analysis-of-allura-red-sunset-yellow-tartrazine-in-mountain-dew-live-wire-at-2-wavelengthsChromatogram
Description
Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow. The dye is banned or restricted in Norway, Finland and Sweden. Obelisc N is a column with very polar characteristics. It contains embedded acidic and basic ionizable groups and can retain Sunset Yellow. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | AmFm pH 3.0 – 70 mM |
| Flow Rate | 0.6 ml/min |
| Detection | UV, 430, 525 nm |
| Class of Compounds | Dye, Acid, Hydrophilic, Ionizable, Zwitterionic |
| Analyzing Compounds | Allura Red, Sunset Yellow, Tartrazine |
HPLC Column Used
Obelisc N, 3.2×50 mm, 5 µm, 100A