HPLC Method for Analysis of Allura Red in Soft Drinks 5

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Chromatogram

Method Parameters

Mobile PhaseMeCN/H2O – 50/50%
BufferAmFm pH 3.0 – 70 mM
Flow Rate0.6 ml/min
DetectionUV, 525 nm
Class of CompoundsDye, Acid, Hydrophilic, Ionizable, Zwitterionic
Analyzing CompoundsAllura Red

HPLC Column Used

Obelisc N, 3.2×50 mm, 5 µm, 100A
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